The prevalence of coronavirus in fecal samples of neonatal calf diarrhea using electron microscopic examination

Authors

  • Abbas Nouri Razi Vaccine and Serum Research Institute, Karaj, Iran
  • Gholamreza Abdollahpour Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Iraj Nowrouzian Department of Surgery,Faculty of Veterinary Medicine,University of Tehran,Tehran, Iran
Abstract:

BACKGROUND:Neonatal calf diarrhea (NCD), also known ascalf scours, is caused by viruses, bacteria, and parasites.Coronavirus is one of the important causes of NCD syndrome.Electron microcopy has been used for demonstration of virusesin fecal samples of diarrheic calves since 1969. OBJECTIVES:The aim of this study was to determine the prevalence ofcoronavirus in fecal samples of neonatal calf diarrhea usingelectron microcopy examination of fecal samples. METHODS:For the purpose of this study, a total of 100 cases (under 2 monthsof age) of diarrheic calves from 25 commercial farms in fourdistricts of East Azerbaijan province of Iran were used. FreshFecal samples using sterile swab were collected from every calfwith clinical diarrhea. All samples were transferred into a sterilecontainer and then were sent to the laboratory to be kept in -20oCfreezer until examination. Electron microscopic examinationwas applied to all fecal samples. Chi-square test was used toanalyze the data. RESULTS:According to the results of this study,coronavirus was present in 15% of cases and a significantdifference was found between the prevalence rate and theseasons (p

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

the prevalence of coronavirus in fecal samples of neonatal calf diarrhea using electron microscopic examination

background:neonatal calf diarrhea (ncd), also known ascalf scours, is caused by viruses, bacteria, and parasites.coronavirus is one of the important causes of ncd syndrome.electron microcopy has been used for demonstration of virusesin fecal samples of diarrheic calves since 1969. objectives:the aim of this study was to determine the prevalence ofcoronavirus in fecal samples of neonatal calf di...

full text

A survey on detection of coronavirus in neonatal calf diarrhea in dairy farms of Iran

BACKGROUND: Bovine coronavirus (BCoV) is a primary cause of neonatal calf diarrhea worldwide, and is also associated with acute diarrhea in adult cattle during the winter season, resulting heavy economic losses to both dairy and beef industry throughout the world. OBJECTIVE: The Objective of the present study was to screen the fecal samples for BCoV collected from diarrhea from six geographic r...

full text

Counterimmunoelectroosmophoresis for detection of neonatal calf diarrhea coronavirus: methodology and comparison with electron microscopy.

A counterimmunoelectroosmophoresis (CIE) technique is described for the detection of calf diarrhea coronavirus antigens in intestinal contents. The antibody reagent was prepared in rabbits against the Nebraska calf diarrhea coronavirus adapted to Vero cells and purified by density gradient centrifugation. The method was applied to intestinal contents of diarrheic and normal calves and compared ...

full text

Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test.

Thirty-four calf and five infant fecal specimens were tested for the neonatal calf diarrhea virus (NCDV) and for the reovirus-like infantile diarrhea agent; respectively. The procedures used were the fluorescent virus precipitin test and immune electron microscopy. Fourteen of the calf stools contained detectable NCDV, and four of the five infant stools contained the reovirus-like human agent. ...

full text

Two genotypes of canine coronavirus simultaneously detected in the fecal samples of dogs with diarrhea.

Sixty-nine fecal samples from diarrheic puppies were examined by reverse transcription-PCR assays for the M and the S genes of canine coronaviruses (CCoVs). The isolates in 10 samples were recognized as CCoV type I, and the isolates in 6 samples were recognized as CCoV type II, while isolates of both genotypes were simultaneously detected in 53 samples.

full text

Investigation of the Therapeutic Efficacy of Sepiolite in Neonatal Calf Diarrhea

The objective of the present study was to determine the therapeutic effectiveness of orally administered sepiolite on neonatal calf with diarrhea. The study was conducted on fifty 1-30 days old public owned calves (40 animals for experiment and 10 animals for control groups), in Afyonkarahisar region, Turkey. Sepiolite was administered orally to all animals in the experiment group for 30 days, ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 8  issue 2

pages  85- 89

publication date 2014-08-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023